epidermal melanocytes hema cell line  (Cell Applications Inc)

Journal: Journal of Synchrotron Radiation

Article Title: Effects of synchrotron-based X-rays and gold nanoparticles on normal and cancer cell morphology and migration

doi: 10.1107/S1600577522012024

Figure Lengend Snippet: Human epidermal melanocytes HEM and human primary melanoma MM418-C1 monolayer 24 h after being exposed to 1000 Gy 90 kVp SBB (width 500 µm). Both cell types are treated with 1 m M AuNPs. The boundaries of the radiation fields are marked with orange lines. (A1) HEM immediately after exposure, (A2) 24 h, (A3) 48 h and (A4) 96 h after exposure. (B1) MM418-C1 immediately after exposure, (B1) 24 h, (B2) 48 h and (B4) 96 h after exposure.

Article Snippet: Moshi Geso), and human colorectal adenocarcinoma SW48 (ATCC ® CCL-231 TM ; Manassas, VA, USA) – along with two different normal cell lines – human epidermal melanocytes HEM (ATCC ® PCS-200–013 TM ; Manassas, VA, USA) and human primary colon epithelial CCD841 CoN (ATCC ® CRL-1790 TM ; Manassas, VA, USA).

Techniques:

Journal: Molecular medicine reports

Article Title: Knockdown of PLCB2 expression reduces melanoma cell viability and promotes melanoma cell apoptosis by altering Ras/Raf/MAPK signals.

doi: 10.3892/mmr.2019.10798

Figure Lengend Snippet: Figure 1. PLCB2 expression in malignant melanoma cell lines. (A) The heatmap analysis was performed by R software with wilcoxTest package (P<0.05 and log2 fold change >1) to illustrate miRNA expression profiles in the GEO database (GSE38290). (B) Volcano plot of DEGs. DEGs with log2 fold change >1.5 are shown in red. GSEA analysis was performed by Broad Institute website to show significantly enriched gene sets in melanoma mainly enriched in (C) cell growth, (D) regula- tion of MAPK cascade and (E) pathways in cancer. (F) The diagram of the PPI network. Co‑expression network of 11 genes. (G) Core protein histogram. The vertical coordinates are the gene names, and the horizontal coordinates are the number of adjacent genes, and the height represents the number of line connected genes. (H) mRNA expression level of PLCB2 in human melanoma cell lines was detected by RT‑PCR. GAPDH was used as a load control. Data are presented as the mean ± standard deviation. **P<0.01 vs. the NHEM cell group. PLCB2, phospholipase C β2; DEGs, differentially expressed genes; PPI‑protein‑protein interaction.

Article Snippet: The normal human epidermal melanocyte (nHeM) cell line was provided by cell applications inc. and cultured in M2 medium.

Techniques: Expressing, Software, Control, Standard Deviation

Journal: South African Journal of Botany

Article Title: Cosmeceutical efficiency by some plant extracts used traditionally for skin care in inhibiting tyrosinase activity in a human epidermal melanocyte (HEM) cell line

doi: 10.1016/j.sajb.2019.06.031

Figure Lengend Snippet: Fig. 2. MTT activity assay of Cassipourea flanaganii, Chenopodium album, Clausena anisata and Rorippa nasturtium-aquaticum on HEM cell lines grown in complete dermal basal medium and supplemented with melanocyte growth supplements expressed as LD50 (μg/mL). Results indicate the mean and standard deviation values with p b .05; n = 3. Letters indicate Fisher's LSD.

Article Snippet: The HEM cell line (PCS-200-013) was purchased from ATCC (American Type Culture Collection) and stored in liquid nitrogen.

Techniques: Activity Assay, Standard Deviation